Journal: Frontiers in Pharmacology
Article Title: Asperosaponin VI suppresses ferroptosis in chondrocytes and ameliorates osteoarthritis by modulating the Nrf2/GPX4/HO-1 signaling pathway
doi: 10.3389/fphar.2025.1539092
Figure Lengend Snippet: AVI suppresses ferroptosis and confers protection to OA cartilage. (A) Fe 2+ content in each group. (B) MDA content in each group. (C–E) Western blot and quantitative analysis of GPX4 and ACSL4 expression levels. (F–G) qRT-PCR analysis of GPX4 and ACSL4 gene expression. (H–L) Western blot and quantitative analysis for Collagen II,MMP13,GPX4 and ACSL4 expression levels. (M–P) qRT-PCR analysis for Collagen II,MMP13,GPX4 and ACSL4 gene expression. (Q–S) ELISA results for IL-6, TNF-α, and PGE2 levels. (T) Fe 2+ content in each group. (U) MDA content in each group. All data are represented as mean ± SD. (n = 3). Compared with the blank group,#p < 0.05,##p < 0.01,# # #p < 0.001,# # # #p < 0.0001; compared with the OA model group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
Article Snippet: The following ELISA kits were used: Rat TNF-α High Sensitivity ELISA Kit (EK382HS-96, MultiSciences, China), Rat IL-6 ELISA Kit (JYM0646Ra, Wuhan Jiyinmei Biotech, China),and Rat Prostaglandin E2 (PGE2) ELISA Kit (JYM0446Ra, Wuhan Jiyinmei Biotech, China).The levels of Fe 2+ and MDA in the cells post-intervention were determined using the Cell Ferrous Iron Colorimetric Assay Kit (E-BC-K881-M, Elabscience, China) and the MDA Colorimetric Assay Kit for Cell Samples (E-BCK0, Elabscience, China).
Techniques: Western Blot, Expressing, Quantitative RT-PCR, Gene Expression, Enzyme-linked Immunosorbent Assay